Evaluation of antioxidant activity of medicinal plants. In the present investigation, the commonly accepted assays viz dpph, frap and abts were used for the evaluation of antioxidant activity of plant extracts. Considering the highly significant medicinal role of antioxidant, in the present investigation, effort is made to find out the antioxidant capacity of hexane extracts of 15 aromatic herbs and spices. Antioxidant capacity of selected plant extracts and their. Antioxidant capacities and phenolic contents of medicinal plants namely usnea longifolia, cetraria nepalensis, par melia minarum, everniastrum nepalense, rhododendron anthopogon and fritillaria delavayi were analyzed via folinciocaltau assay, ferric reducing activity power assay and 2,2diphenyl1picrylhydrazyl assay. Antibacterial, antifungal and antioxidant activities of some medicinal plants 2146 pak. Photoprotective potential of medicinal plants from cerrado biome brazil in relation to phenolic content and antioxidant activity. With the dpph test the ability of a compound to act as a donor for hydrogen atoms or the antioxidant activity of some medicinal plants 200 table 2. Screening of medicinal plant extracts for antioxidant activity. The antioxidant activity was estimated using 2, 2di phenyl1picrylhydrazyl dpph as free radical scavenger. Effect of extraction solventtechnique on the antioxidant. The many number of medicinal plants are used in the cellular and metabolic.
Research article open access antioxidant activity, total phenolic and total. Estimation of phytochemical content and antioxidant. A large number of medicinal plants and their purified constituents have shown beneficial therapeutic potentials. The most common and widely distributed group of phenolic compounds in plants is flavonoids. The aim of the present study was to assess antioxidant, antibacterial and antitumor activity of ethanolic extract of the psidium guajava leaves. Antioxidant activity of medicinal spices and aromatic herbs. Several methods have been developed to assay the antioxidant activity of herbal and plant extracts. The antioxidant activity of the 90 plant extracts was determined by 2, 2 diphenyl1picrylhydrazyl dpph assay. Evaluation of antioxidant activity of two important memory enhancing. A study of antimicrobial activity of few medicinal herbs. Pdf antioxidants or inhibitors of oxidation are compounds which retard or prevent the oxidation and in general prolong the life of the oxidizable.
Study of total phenolic and flavonoid content, antioxidant. Chemistry and antioxidant activity of plants containing. Photoprotective potential of medicinal plants from cerrado. Green tea, oligomeric procyanidins from grape seed and pine bark, bilberry, and ginkgo exhibited taa in the range of 5. Moisture, ash content and acid insoluble ash of different medicinal plants. Phytochemical investigation and in vitro antioxidant activity. The table 2, 3, and 4 shows the antioxidant activities of the ethanol, methanol and hexane extracts of six. Generally higher extract yields, phenolic contents and plant material antioxidant activity were obtained using aqueous organic solvents, as compared to the respective absolute organic solvents. Introduction the many number of medicinal plants are used in the cellular and metabolic disease. The present study therefore was conducted with the main objective of investigating the most effective solventtechnique for extracting potent antioxidant compounds, especially phenolics from different parts of selected medicinal plants native to pakistan. Estimation of phytochemical content and antioxidant activity. Minimal inhibitory concentration of metanoic extracts ranged from 6. Evaluation of antioxidant activity of medicinal plant 885 figure 2. Antimicrobial activity of three medicinal plants artemisia indica, medicago falcata and tecoma stans tahir javid 1, muhammad adnan1, akash tariq1,basreen akhtar, riaz ullah2, naser m.
There is an increasing interest in natural antioxidants e. The major plant compounds characterized by antioxidant activity are polyphenols. The in vitro screening for acetylcholinesterase inhibition. Medicinal plants used in the traditional medicine are wellknown significant sources of natural antioxidants. Antioxidant activity and total phenolic content of some medicinal plants in urticaceae family. Antioxidant and antimicrobial properties of five medicinal. There has been increasing interest in the research on flavonoids from plant sources because of their versatile health benefits reported in various epidemiological studies. However, there is little information available concerning antioxidant activity of medicinal plants growing wild in northeastern iran. The type of extragent significantly influenced the efficiency of the polyphenol extraction and the anti. Wine has been recognized for its antioxidant activity kinsella et al. Plantderived products such as polyphenols, terpene. The results suggest that phenolic compounds are the significant contributors to the antioxidant activity of the medicinal plants studied. Guava, antitumor, medicinal plants, functional foods 1. In most of the plants the tpc and the antioxidant activity of the ethanol extracts were significantly higher p of water alone extracts.
Antioxidant activity, total phenolic and flavonoid contents. The aim of this work was to study the structure activity relationships of the antioxidant activity of natural coumarins isolated from four korean medicinal plants 117 and four purchased coumarins 1821. Five libyan medicinal plants thapsia garganica, hammada scoparia, euphorbia serrata, hyoscya mus albus and retama rateam were selected to evaluate their biological activities. Antioxidant, antibacterial and antitumor activity of. At the same time, the antioxidant activities of some essential oils, measured through several methodologies, are presented and compared. Some indian traditional medicinal plants w ith antioxidant. Antioxidant activity by dpph radical scavenging method of. Antioxidant properties of several medicinal plants. Research article open access antioxidant activity, total. May 26, 2009 the aim of this work was to study the structureactivity relationships of the antioxidant activity of natural coumarins isolated from four korean medicinal plants 117 and four purchased coumarins 1821. The plants act as a source of antioxidants or free radical scavengers, the antilipid peroxidation and immune modulator. Caffea arabica showed greater antioxidant activity measured as scavenging of dpph, superoxide radicals, reducing power and inhibition of microsomal lipid peroxidation. Antioxidant activity, total phenolic and flavonoid.
Extract, free radical, antioxidant activity, medicinal plant. The wine we tested had significantly lower free radical activity than 86% of the medicinal plants tested with 20% of the plants also higher in superoxide scavenging. Antioxidant activity of some common medicinal plants. The bioavailability, metabolism, and biological activity of flavonoids.
Antioxidant properties of medicinal plants from peru. These compounds have been used as antioxidant, antcarcinogenic, antifungal, antibacterial, antispasmodic, antiinflammatory and antidiabetic. Dpph stable free radical method is a sensitive way to determine the antioxidant activity of plant extracts, thus it was considered important to screen the ocimum tenuiflorum extracts for antioxidant activity against. Pharmacological evaluation of bangladeshi medicinal plants for antioxidant activity m. Introduction experimental evidence suggests that free radicals and reactive oxygen spec ies ros can be involved in high number. The meoh extracts of the 2 most antimicrobial active plants, t. Phytochemical investigation and in vitro antioxidant activity of an. These plants exhibit strong antioxidant activity that can explain their uses in traditional medicines in the past as well as prese nt. Several medicinal plants rasayana have also been extensively used in the indian traditional ayurveda system of medicine for the treatment of number of diseases18. Journal of medicinal plant research vol 624pp 40824086 26 june, 2012. Some of these plants have shown potent antioxidant activity16,7. Their total phe nolic and flavanoid contents were assessed. With the emergence of multiple strains of antibiotic resistance microorganism, great interest has been generated in search for potential compounds from plants for therapeutic, medicinal, aromatic and aesthetic uses 2,3. The proximate compositions of different medicinal plants are presented in tables 2 and 3.
Antioxidant activity exhibited by medicinal plants. Plants contain a wide variety of free radical scavenging molecules, such as polyphenols, dietary glutathione, vitamins and endogenous metabolites. This oxidative damage has an important etiological role in aging and the development of diseases like cancer, atherosclerosis, and other inflammatory disorders. Results showed that both of ethanolic and water extracts of some plant possessed high cytotoxic and antioxidant activities and inhibited the cell growth of cancer cells. The free radical scavenger activity was higher for the polar extracts. Dpph scavenging activity has been used by various researchers as a quick and reliable parameter to assess the in vitro antioxidant activity of crude plant extracts. The phytochemical analysis of these medicinal plants have revealed a large number of compounds including flavonoids and phenolics which have shown potent antioxidant activity. The moisture content of the plant samples was found to. Singh 1, poonam singh 1 scientist, division of toxicology, csircentral drug research institute, lucknow, u.
Screening of in vitro antioxidant activity of methanolic. Pdf in vitro antioxidant and antiproliferative activity. In this study, 11 medicinal plants were analysed for their proximate composition and antioxidant activity. Oxygen radical absorbance capacity orac and total polyphenol content of 25 bulgarian medicinal plants subjected to water or 80 % acetone extractions were investigated and compared. For the past three decades, antioxidants are used to treat complex disorders such as cancer and alzheimers disease. Different parameters studied include phenolic contents, moisture, ash, crude fiber, fats and waxes. The main active antioxidant compounds isolated from. Witch hazel, propolis epid, artichoke, and hawthorn afforded.
Antioxidant activity of medicinal plants in di erent biological substrates. Study of total phenolic and flavonoid content, antioxidant activity and antimicrobial properties of medicinal plants volume 1 issue 1 2014 sarabjot kaur,1 poonam mondal2 1department of pharmaceutical sciences, assam university, india 2department of biotechnology, lovely professional university, india. Antimicrobial and antioxidant activities of some nigerian. Page 21 dpph free radical scavenging activity of some leafy vegetables used by tribals of odisha, india.
Gangavaram maheshwar reddy,visweswaran rao dolly sarma etal evaluation of antioxident activity index by the 2,2 diphenyl 1 picryl hydrazyl method of 40 medicinal plants. The ascorbic acid, carotenoids and total phenol contents with antioxidant activity were also determined. Previous studies showed that a wide range of medicinal plants exerted antioxidant activity. Evaluation of antioxidant activity of medicinal plant extracts. Antioxidant activity and phenolic content of some medicinal. The antioxidant activity of these two medicinal plants was evaluated by measuring reducing ability, free radical scavenging activity by dpph and hydrogen peroxide methods.
Since flavonoids are directly associated with human dietary ingredients and health, there is need to evaluate structure and function relationship. Table 1 gives a list of medicinal plants analysed along with parts used and medicinal uses. Acetylcholinesterase inhibition and antioxidant activity of some medicinal plants for treating neuro degenarative disease abdelaaty a. Shahabimajd2 1department of medicinal chemistry, faculty of pharmacy, mazandaran university of medical sciences, sari, iran. It is evident that the extracts of the plant samples have shown varied inhibitory activity. Page 21 dpph free radical scavenging activity of some leafy vegetables used by tribals of odisha, india rajani kanta sahu 1, manoranjan kar 2, rasmirani routray 3, 1. Antioxidant properties of indian medicinal plants wiley online library. Abstract identification and characterization of natural products with antioxidant and antiproliferative activity has received much interest over the past few years. Minimal bactericidal concentration of metanoic extracts ranged from 12. Antioxidant activity of selected medicinal plants journal. Antioxidant activity exhibited by medicinal plants, vegetables and fruits from north of iran reyhaneh sariri. All the tested plants depicted the antioxidant activity. The powder samples and methanol extract of 11 medicinal plants were subjected to analysis of proximate composition and measurement of antioxidant activity.
Total phenolic and flavonoid contents and antioxidant activity of four iranian herbs lamiaceae were investigated. The antioxidant properties, determined with dpph and abts scavenging assays as. The airdried plant samples were into uniform powder using milling machine separately. Antioxidant activity screening assays implemented here is % dpph radical scavenging activity rsa. Infusions of all studied plants have shown antioxidant activity, though there was a large diversity between the results. Antioxidant activity, total phenolic and flavonoid contents of selected medicinal plants used for liver diseases and its classification with chemometrics harto widodo 1,2, sismindari sismindari3, widya asmara 4, abdul rohman 3,5 1centre study of biotechnology, gadjah mada university, yogyakarta, indonesia. Acetylcholinesterase, alzheimer, antioxidant, antilipidperoxidation, medicinal plants introduction the investigation of plants used as remedies in traditional folk medicine can be as useful tool to identify several biologically active molecules from the 250 000 higher plant species testai, et al. Dpph radical scavenging ability is widely used as an index to evaluate the antioxidant potential of medicinal plants. Antibacterial, antifungal and antioxidant activities of. The antioxidant activity of polyphenols is accredited to their redox properties i.
Medicinal plants derived natural antioxidants, which are in the form of raw extracts andor chemical constituents, are very efficient to block the process of oxidation by neutralizing free radicals 3. View enhanced pdf access article on wiley online library html view download pdf for offline viewing. The antioxidant activity of methanol extracts of thyme thymus daenensis celak. Libyan capparis spinose has good antioxidant activity.
Several herbs and spices have been reported to show antioxidant activity. The interest in the exogenous plant antioxidants was first evoked by. Review significance of antioxidant potential of plants and. Acetylcholinesterase inhibition and antioxidant activity of. It is believed that twothirds of the worlds plant species have medicinal importance, and almost all of these have excellent antioxidant potential 3. Keeping the above in view the present chapter focuses on the i diversity of antioxidant phytochemicals in medicinal.
Antioxidant capacity and phenolic content of some nepalese. Frap assay was done according to benzie and strain procedure 40. Medicinal plantsderived natural antioxidants, which are in the form of raw extracts andor chemical constituents, are very efficient to block the process of oxidation by neutralizing free radicals 3. Acetylcholinesterase inhibition and antioxidant activity. In the water extracts most of the plants showed values around 90%. Antioxidant capacities and phenolic contents of medicinal plants namely usnea longifolia, cetraria nepalensis, parmelia minarum, everniastrum nepalense, rhododendron anthopogon and fritillaria delavayi were analyzed via folinciocaltau assay, ferric reducing activity power assay and 2,2diphenyl1picrylhydrazyl assay. The free radical scavenging activity against 1,1diphenyl2picryl hydrazyl dpph was evaluated during the course of work.
The total phenolic contents and flavonoid contents were also determined. These potential herbs should be further studied for their active substances and other biological activities. Nitric oxide scavenging activity, antioxidant activity, active nitrogen species, sodium nitroprusside. The medicinal plants research on antioxidants, antiradiation, antilipid peroxidation and free radicals scavenging plants. Evaluation of antioxidant activity of medicinal plant. The antioxidant properties of methanol extracts of 12 indian medicinal plants, traditionally used in dis ease areas that probably involve free radical mechanisms.
The study showed that the plant revealed an antioxidant activity 80% with ic 50 0. Antioxidant activity of medicinal and aromatic plants. Effect of extraction solventtechnique on the antioxidant activity of selected medicinal plant extracts. Antioxidant activity of fourteen herbal plants in thailand. Pdf antioxidant activity in vitro of peruvian medicinal. The most practical way to fight degenerative diseases is to increase antioxidant activity in our body and that could be achieved by consumption of vegetables, fruits or edible plants. Antibacterial and antioxidant activity of traditional. Natural compounds especially those that are derived from medicinal plants provide a large number of antioxidants useful for humans. Antioxidant activity in medicinal plants associated with the. Rahman2 1department of pharmacy, jahangirnagar university, dhaka, bangladesh. Antioxidant activities of coumarins from korean medicinal. Aug 19, 2010 in the present study, a brief description of the mechanism involved in the majority of techniques used for the evaluation of antioxidant activity of essential oils is given. Ethiopia is one of the developing countries which have enormous diversity of plants.
Abdelsalam3 1 department of botany, kohat university of science and technology, kohat, pakistan. According to the folk experts in this field, there are so many cases that have benefited from treatment by these herbal medicinal plants after they lost their faith in treatment by the medicament. A study of antimicrobial activity of few medicinal herbs parastoo karimi alavijeh, parisa karimi alavijeh and devindra sharma j. Antioxidant activity, phenol and flavonoid contents of some. The antioxidants compounds like ascorbic acid, total phenols and tannins were also evaluated in these plants. Pharmacological evaluation of bangladeshi medicinal plants. Antioxidant properties of medicinal plants from peru 73. Effect of extraction solventtechnique on the antioxidant activity of selected medicinal plant extracts bushra sultana 1, farooq anwar 1, and muhammad ashraf 2 1 department of chemistry and biochemistry, university of agriculture, faisalabad38040, pakistan 2 department of botany, university of agriculture, faisalabad38040, pakistan.
The assays employed were ferric reducing antioxidant power, trolox equivalent antioxidant. Evaluation of antioxidant activity of two important memory. Rosemary, sage, thyme, nutmeg, turmeric, white pepper, chilli, pepper, ginger, garlic and several medicinal plants extracts exhibit antioxidant activity 41,42,43,44. The present investigation suggests that medicinal plants which possess good antioxidant potential are the best supplements for the diseases associated with oxidative stress. Antioxidant activity, phenol and flavonoid contents of some selected iranian medicinal plants f. Various herbs and spices have been reported to exhibit antioxidant activity, including ocimum sanctum, piper cubeba linn. Pdf modern food habits, visavis lifestyle, has started taking its toll in the form of obesity, depression, autoimmune diseases, spondylosis. The potential antioxidant activity of the extract was assessed on the basis of the scavenging. Screening of in vitro antioxidant activity of methanolic leaf. Antioxidant properties of several medicinal plants growing. In vitro nitric oxide scavenging activity of methanol.
Commonly used medicinal plant extracts with standardized content of polyphenols were investigated for their total antioxidant activity taa. J, in vitro antioxidant activity of coumarin compounds by dpph, superoxide and nitric oxide free radical scavenging methods journal of advanced. Understanding their mechanism of action and therapeutic efficacy, 2012. The antioxidant activity of these two medicinal plants was evaluated by measuring reducing ability, free radical scavenging activity by dpph and hydrogen. Antioxidant activity of some common medicinal plants impact factor. Pdf antioxidant activity and total phenolic content of some. Antibacterial and antioxidant activities of 8 aromatic plants from the balkan peninsula were studied. Pdf perspective of dietetic and antioxidant medicinal plants. Keeping the above in view the present chapter focuses on the i.